GQ alpha/g11 alpha (C-Term) Antikörper

Produktnummer ABIN470875

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch in IP und WB. Er zeigt eine Reaktivität gegenüber Fledermaus, Huhn, Rind (Kuh), Hund, Ziege, Hamster, Pferd, Human, Affe, Maus, Schwein, Kaninchen, Ratte, Xenopus laevis und Zebrafisch (Danio rerio).

Kurzübersicht für GQ alpha/g11 alpha (C-Term) Antikörper (ABIN470875)

Target: GQ alpha/g11 alpha

Reaktivität: Fledermaus, Huhn, Rind (Kuh), Hund, Ziege, Hamster, Pferd, Human, Affe, Maus, Schwein, Kaninchen, Ratte, Xenopus laevis, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Immunoprecipitation (IP), Western Blotting (WB)

Produktdetails

Bindungsspezifität: C-Term

Spezifität: Specific for human Gq alpha and G11 alpha. Does not recognize other non-pertussis toxin-sensitive G proteins. Species cross-reactivity: Mouse and human.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Stickleback, Pufferfish, Zebrafish, Blood fluke, Sea anemone, Tick (100%) Water flea (90%).

Aufreinigung: Purified

Immunogen: KLH-conjugated, synthetic peptide (QLNLKEYNLV) corresponding to amino acids 350- 359 of G11 alpha or 344-353 of Gq alpha. This sequence is highly conserved among species. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Stickleback, Pufferfish, Zebrafish, Blood fluke, Sea anemone, Tick (100%), Water flea (90%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IP (1:500 - 1:2000), WB

Usage: Suitable for use in Western Blot analysis and Immunoprecipitation. Immunoprecipitation: 1:500-1:2000 (detects Gq alpha and G11 alpha in 20 μg of mouse brain microsomes and membranes, detected Gq/11 a in 10 μg of mouse 3T3/NIH fibroblast soluble plasma membranes.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Antigendetails

Target: GQ alpha/g11 alpha